PSMC3 antibody

Catalog No. ABIN2856692

Product Details anti-PSMC3 Antibody

Target: PSMC3 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMC3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Characteristics: Rabbit polyclonal antibody to PSMC3 (proteasome (prosome, macropain) 26S subunit, ATPase, 3)
PSMC3 antibody [N1C2]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PSMC3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PSMC3

Target: PSMC3 (Proteasome (Prosome, Macropain) 26S Subunit, ATPase, 3 (PSMC3))

Alternative Name: proteasome 26S subunit, ATPase 3 (PSMC3 Products)

Synonyms: TBP1 antibody, TBP-1 antibody, wu:fb39a02 antibody, zgc:86713 antibody, psmc3 antibody, MGC89608 antibody, PSMC3 antibody, NV15940 antibody, 23.m06430 antibody, DDBDRAFT_0186002 antibody, DDBDRAFT_0232967 antibody, DDB_0186002 antibody, DDB_0232967 antibody, psmc3-b antibody, tbp10 antibody, psmc3-a antibody, proteasome 26S subunit, ATPase 3 antibody, proteasome (prosome, macropain) 26S subunit, ATPase 3 antibody, 26S protease regulatory subunit 6A antibody, 26S protease regulatory subunit 6a antibody, 26S protease regulatory subunit 6A-B antibody, 26S protease regulatory subunit 6A, putative antibody, proteasome 26S subunit, ATPase 3 S homeolog antibody, nth-like DNA glycosylase 1 S homeolog antibody, PSMC3 antibody, Psmc3 antibody, HCAG_00069 antibody, GL50803_4365 antibody, EDI_316920 antibody, CpipJ_CPIJ006888 antibody, CpipJ_CPIJ006889 antibody, CpipJ_CPIJ010864 antibody, prs6a antibody, psmc3 antibody, LOC100115023 antibody, BBOV_IV004580 antibody, CGB_M2140W antibody, psmC3 antibody, LOC100637883 antibody, psmc3.S antibody, nthl1.S antibody

Background: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the ATPase subunits, a member of the triple-A family of ATPases that have chaperone-like activity. This subunit may compete with PSMC2 for binding to the HIV tat protein to regulate the interaction between the viral protein and the transcription complex. A pseudogene has been identified on chromosome 9.

Cellular Localization: Cytoplasm (Potential) , Nucleus

Molecular Weight: 49 kDa

Gene ID: 5702

UniProt: P17980

Pathways: Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Ubiquitin Proteasome Pathway