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RPA1 antibody (C-Term)

Name: RPA1 antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2856633
Price: 637.78 USD
Availability: InStock

RPA1 Reactivity: Human WB, IF, IP, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample (50 ug of whole cell lysate) A: mouse liver 7. (RPA1 antibody (C-Term))

IP Image Immunoprecipitation of RPA70 protein from 293T whole cell extracts using 5 μg of RPA70 antibody [C1C3], Western blot analysis was performed using RPA70 antibody [C1C3], EasyBlot anti-Rabbit IgG was used as a secondary reagent. (RPA1 antibody (C-Term))

IHC-P Image RPA70 antibody [C1C3] detects RPA1 protein at nucleus in mouse brain by immunohistochemical analysis. (RPA1 antibody (C-Term))

IHC

IHC-P Image RPA70 antibody [C1C3] detects RPA70 protein at cytoplasm and nucleus in human lung papillary adenocarcinoma by immunohistochemical analysis. (RPA1 antibody (C-Term))

IHC

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using RPA 70 kDa subunit , antibody at 1:100 dilution. (RPA1 antibody (C-Term))

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPA 70 kDa subunit , antibody at 1:200 dilution. (RPA1 antibody (C-Term))

WB Image RPA70 antibody detects RPA70 protein by western blot analysis. (RPA1 antibody (C-Term))

7 images

Catalog No. ABIN2856633

Datasheet as PDF

Target See all RPA1 Antibodies

RPA1 (Replication Protein A1, 70kDa (RPA1))
Binding Specificity
16

9

6

4

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
Reactivity
75

24

14

4

3

3

2

2

2

2

2

2

2

2

1
Human
Host
62

12

1

1
Rabbit
Compatible Secondaries
Clonality
61

15
Polyclonal
Conjugate
45

4

4

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RPA1 antibody is un-conjugated
Application
63

27

20

14

14

13

13

12

9

7

3

2

2

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse

Characteristics

Rabbit Polyclonal antibody to RPA70 (replication protein A1, 70 kDa)
RPA70 antibody [C1C3]

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RPA70. The exact sequence is proprietary.

Isotype

IgG

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RPA1 Reactivity: Human WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: Mouse liver , 293T , A431 , HeLa , HepG2

Restrictions

For Research Use only
Format

Liquid

Concentration

0.4 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Target

RPA1 (Replication Protein A1, 70kDa (RPA1))

Alternative Name

replication protein A1 (RPA1 Products)

Background

Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair, it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

Cellular Localization: Nucleus

Molecular Weight

68 kDa

Gene ID

6117

UniProt

P27694

Pathways

Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA