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TJP1 antibody (N-Term)

Name: TJP1 antibody (N-Term)
Brand: antibodies-online
SKU: ABIN2856624
Price: 609.18 USD
Availability: InStock

KO Validated TJP1 Reactivity: Human WB, IP, IF, ICC Host: Rabbit Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:H1299 5% SDS PAGE antibody diluted at 1:3000 (TJP1 antibody (N-Term))

IHC-P Image Immunohistochemical analysis of paraffin-embedded H661 xenograft, using ZO-1, antibody at 1:250 dilution. (TJP1 antibody (N-Term))

IHC

WB Image Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ZO-1 antibody [N1N2], N-term , diluted at 1:500. (TJP1 antibody (N-Term))

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse intestine by immunohistochemical analysis. (TJP1 antibody (N-Term))

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects TJP1 protein at junction by confocal immunofluorescent analysis. (TJP1 antibody (N-Term))

IHC-P Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane and cytoplasm in mouse testis by immunohistochemical analysis. (TJP1 antibody (N-Term))

IHC

ICC/IF Image ZO-1 antibody [N1N2], N-term detects ZO-1 protein at cell membrane by immunofluorescent analysis. (TJP1 antibody (N-Term))

Identification of SLC26A3 as a CFTR and ZO-1 binding partner and effects on their interaction. (TJP1 antibody (N-Term))

8 images

(1 reference)

Catalog No. ABIN2856624

Datasheet as PDF

Target See all TJP1 Antibodies

TJP1 (Tight Junction Protein 1 (TJP1))
Binding Specificity
16

6

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
57

38

37

20

18

9

6

5

4

4

2

2

1

1

1

1

1

1

1

1
Human
Host
53

3

2

1

1
Rabbit
Compatible Secondaries
Clonality
55

5
Polyclonal
Conjugate
36

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TJP1 antibody is un-conjugated
Application
49

20

17

15

14

13

12

10

10

8

6

3

2

1
Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity

Human, Mouse

Characteristics

Rabbit Polyclonal antibody to ZO-1 (tight junction protein 1 (zona occludens 1))
ZO-1 antibody [N1N2], N-term

Purification

Purified by antigen-affinity chromatography.

Grade

KO Validated

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human ZO-1. The exact sequence is proprietary.

Isotype

IgG

Top Product

Discover our top product TJP1 Primary Antibody
anti-Tight Junction Protein 1 (TJP1) (AA 1551-1702) antibody
TJP1 Reactivity: Human, Mouse, Rat, Pig, Cow WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (C-Term) antibody
TJP1 Reactivity: Human, Mouse, Rat, Pig, Cow, Dog, Guinea Pig, Rabbit, Horse, Monkey, Chicken, Avian, Cat, Donkey, Goat, Hamster, Sheep WB, IF, IHC (p), IHC (fro) Host: Goat Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (Internal Region) antibody
Verified TJP1 Reactivity: Human ELISA, IF Host: Goat Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1600-1700) antibody
TJP1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivity: Human WB, IHC, ELISA, FACS, FM Host: Rabbit Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivity: Human, Mouse, Rat, Pig WB, IHC, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1178-1527) antibody
TJP1 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1570-1600), (C-Term) antibody
TJP1 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB15419 unconjugated

anti-Tight Junction Protein 1 (TJP1) antibody
TJP1 Reactivity: Mouse WB, IHC, IP Host: Rat Monoclonal unconjugated

anti-Tight Junction Protein 1 (TJP1) (AA 1-190) antibody
TJP1 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment

Positive Control: HeLa
Validation: KO/KD

Restrictions

For Research Use only
Format

Liquid

Concentration

0.22 mg/mL

Buffer

1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Zhang, Heruth, Wu, Zhang, Nsumu, Shortt, Li, Jiang, Wang, Friesen, Li, Ye: "Functional characterization of SLC26A3 c.392C>G (p.P131R) mutation in intestinal barrier function using CRISPR/CAS9-created cell models." in: Cell & bioscience, Vol. 9, pp. 40, (2019) (PubMed).
Target

TJP1 (Tight Junction Protein 1 (TJP1))

Alternative Name

tight junction protein 1 (TJP1 Products)

Synonyms

ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.

Cellular Localization: Cell membrane, Peripheral membrane protein, Cytoplasmic side , Cell junction , tight junction

Molecular Weight

195 kDa

Gene ID

7082

UniProt

Q07157

Pathways

Carbohydrate Homeostasis, Cell-Cell Junction Organization