RAMP2 antibody (C-Term)

Catalog No. ABIN2856615

Product Details anti-RAMP2 Antibody

Target: RAMP2 (Receptor (G Protein-Coupled) Activity Modifying Protein 2 (RAMP2))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to RAMP2 (receptor (G protein-coupled) activity modifying protein 2)
RAMP2 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human RAMP2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RAMP2 antibody (ABIN2856615)

Edvinsson, Grell, Warfvinge: "Expression of the CGRP Family of Neuropeptides and their Receptors in the Trigeminal Ganglion." in: Journal of molecular neuroscience : MN, Vol. 70, Issue 6, pp. 930-944, (2020) (PubMed).

Blom, Giove, Pong, Blute, Eldred: "Evidence for a functional adrenomedullin signaling pathway in the mouse retina." in: Molecular vision, Vol. 18, pp. 1339-53, (2012) (PubMed).

Target Details for RAMP2

Target: RAMP2 (Receptor (G Protein-Coupled) Activity Modifying Protein 2 (RAMP2))

Alternative Name: receptor activity modifying protein 2 (RAMP2 Products)

Synonyms: RAMP2 antibody, zgc:174936 antibody, receptor activity modifying protein 2 antibody, receptor (G protein-coupled) activity modifying protein 2 antibody, receptor (calcitonin) activity modifying protein 2 antibody, RAMP2 antibody, ramp2 antibody, Ramp2 antibody

Background: The protein encoded by this gene is a member of the RAMP family of single-transmembrane-domain proteins, called receptor (calcitonin) activity modifying proteins (RAMPs). RAMPs are type I transmembrane proteins with an extracellular N terminus and a cytoplasmic C terminus. RAMPs are required to transport calcitonin-receptor-like receptor (CRLR) to the plasma membrane. CRLR, a receptor with seven transmembrane domains, can function as either a calcitonin-gene-related peptide (CGRP) receptor or an adrenomedullin receptor, depending on which members of the RAMP family are expressed. In the presence of this (RAMP2) protein, CRLR functions as an adrenomedullin receptor. The RAMP2 protein is involved in core glycosylation and transportation of adrenomedullin receptor to the cell surface.

Cellular Localization: Membrane, Single-pass type I membrane protein

Molecular Weight: 20 kDa

Gene ID: 10266

UniProt: O60895

Pathways: cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling