H2AFZ antibody (N-Term)

Catalog No. ABIN2856590

Product Details anti-H2AFZ Antibody

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFZ antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Human, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to Histone H2A.Z (H2A histone family, member Z)
Histone H2A.Z antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H2A.Z. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Jurkat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.52 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-H2AFZ antibody (ABIN2856590)

Piquet, Le Parc, Bai, Chevallier, Adam, Polo: "The Histone Chaperone FACT Coordinates H2A.X-Dependent Signaling and Repair of DNA Damage." in: Molecular cell, Vol. 72, Issue 5, pp. 888-901.e7, (2018) (PubMed).

Target Details for H2AFZ

Target: H2AFZ (H2A Histone Family, Member Z (H2AFZ))

Alternative Name: H2A histone family member Z (H2AFZ Products)

Synonyms: H2A.Z-1 antibody, H2A.z antibody, H2A/z antibody, H2AZ antibody, H2A.Z antibody, H2a.z-1 antibody, H2AFV antibody, H2Az antibody, XH2AZ antibody, h2a.zl1 antibody, h2a.zl2 antibody, H2A histone family member Z antibody, H2A histone family, member Z antibody, H2A histone family member Z L homeolog antibody, histone H2A.Z antibody, H2AFZ antibody, H2afz antibody, h2afz.L antibody

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality.

Cellular Localization: Nucleus

Molecular Weight: 14 kDa

Gene ID: 3015

UniProt: P0C0S5

Pathways: Telomere Maintenance