GSTA1 antibody

Catalog No. ABIN2856555

Product Details anti-GSTA1 Antibody

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GSTA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Based on sequence homology, this antibody may cross react with other GSTA family members.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to GSTA1 (glutathione S-transferase alpha 1)
GSTA1 antibody

Purification: Affinity purified by Protein A.

Immunogen: Recombinant protein corresponding to human GSTA1

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse liver , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 6 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for GSTA1

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Alternative Name: glutathione S-transferase alpha 1 (GSTA1 Products)

Background: Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. These enzymes function in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding these enzymes are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of some drugs. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-tranferase belonging to the alpha class. The alpha class genes, located in a cluster mapped to chromosome 6, are the most abundantly expressed glutathione S-transferases in liver. In addition to metabolizing bilirubin and certain anti-cancer drugs in the liver, the alpha class of these enzymes exhibit glutathione peroxidase activity thereby protecting the cells from reactive oxygen species and the products of peroxidation.

Cellular Localization: Cytoplasm

Molecular Weight: 26 kDa

Gene ID: 2938

UniProt: P08263