ZWINT antibody (Center)

Catalog No. ABIN2856493

Product Details anti-ZWINT Antibody

Target: ZWINT (ZW10 Interactor (ZWINT))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZWINT antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Rabbit Polyclonal antibody to ZWINT (ZW10 interactor)
ZWINT antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ZWINT. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , Molt-4 , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ZWINT antibody (ABIN2856493)

Zhou, Wang, Zheng, Teng, Chen: "Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2." in: Nature communications, Vol. 7, pp. 10151, (2016) (PubMed).

Target Details for ZWINT

Target: ZWINT (ZW10 Interactor (ZWINT))

Alternative Name: ZWINT (ZWINT Products)

Synonyms: ZWINT antibody, HZwint-1 antibody, KNTC2AP antibody, ZWINT1 antibody, 2010007E07Rik antibody, 2600001N01Rik antibody, D10Ertd749e antibody, Zwint-1 antibody, Sip30 antibody, ZW10 interacting kinetochore protein antibody, ZW10 interactor antibody, ZWINT antibody, Zwint antibody

Background: This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Nucleus , Kinetochore

Molecular Weight: 31 kDa

Gene ID: 11130