EIF3H antibody

Catalog No. ABIN2856455

Product Details anti-EIF3H Antibody

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3H antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to EIF3S3 (eukaryotic translation initiation factor 3, subunit H)
EIF3 gamma antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human eIF3H / EIF3S3. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , H1299 , HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for EIF3H

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Alternative Name: eukaryotic translation initiation factor 3 subunit H (EIF3H Products)

Background: Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.

Cellular Localization: Cytoplasm

Molecular Weight: 40 kDa

Gene ID: 8667

UniProt: O15372

Pathways: Ribonucleoprotein Complex Subunit Organization