MAF1 antibody (Center)

Catalog No. ABIN2856430

Product Details anti-MAF1 Antibody

Target: MAF1 (MAF1 Homolog (MAF1))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Characteristics: Rabbit Polyclonal antibody to MAF1 (MAF1 homolog (S. cerevisiae))
MAF1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , Molt-4 , GL261 , BCL-1 , Raw264.7 , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for MAF1

Target: MAF1 (MAF1 Homolog (MAF1))

Alternative Name: MAF1 (MAF1 Products)

Synonyms: 1110068E11Rik antibody, AU042856 antibody, maf1 antibody, MAF1 homolog, negative regulator of RNA polymerase III antibody, MAF1 homolog, negative regulator of RNA polymerase III L homeolog antibody, MAF1 antibody, Maf1 antibody, maf1.L antibody

Background: This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression.

Cellular Localization: Nucleus

Molecular Weight: 29 kDa

Gene ID: 84232