RAD21 antibody (Center)

Catalog No. ABIN2856242

Product Details anti-RAD21 Antibody

Target: RAD21 (RAD21 Homolog (RAD21))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Characteristics: Rabbit Polyclonal antibody to RAD21 (RAD21 homolog (S. pombe))
RAD21 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human RAD21. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: K562 , THP-1 , HL-60 , NIH-3T3 , JC , BCL-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RAD21 antibody (ABIN2856242)

Ochiai, Hayashi, Umeda, Yoshimura, Harada, Shimizu, Nakano, Saitoh, Liu, Yamamoto, Okamura, Ohkawa, Kimura, Nikaido: "Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells." in: Science advances, Vol. 6, Issue 25, pp. eaaz6699, (2020) (PubMed).

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: RAD21 (RAD21 Products)

Synonyms: rad21 antibody, wu:fb55d02 antibody, wu:fb93f04 antibody, CDLS4 antibody, HR21 antibody, HRAD21 antibody, MCD1 antibody, NXP1 antibody, SCC1 antibody, hHR21 antibody, mKIAA0078 antibody, hr21 antibody, mcd1 antibody, nxp-1 antibody, nxp1 antibody, scc1 antibody, xrad21 antibody, RAD21 cohesin complex component antibody, RAD21 cohesin complex component a antibody, RAD21 cohesin complex component L homeolog antibody, RAD21 cohesin complex component S homeolog antibody, R-spondin 2 antibody, RAD21 antibody, rad21a antibody, rad21.L antibody, Rad21 antibody, rad21.S antibody, RSPO2 antibody

Background: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.

Cellular Localization: Nucleus

Molecular Weight: 72 kDa

Gene ID: 5885

Pathways: Positive Regulation of Endopeptidase Activity