PDK1 antibody

Catalog No. ABIN2856238

Product Details anti-PDK1 Antibody

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDK1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Based on sequence homology, this antibody may cross react with other PDK family members.

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to PDK1 (pyruvate dehydrogenase kinase, isozyme 1)
PDK1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Pyruvate dehydrogenase kinase 1 / PDK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLaS3 , Molt-4 , human PDK1 transfected 293T cells

Validation: Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PDK1

Target: PDK1 (Pyruvate Dehydrogenase Kinase 1 (PDK1))

Alternative Name: pyruvate dehydrogenase kinase 1 (PDK1 Products)

Background: Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 49 kDa

Gene ID: 5163

UniProt: Q15118

Pathways: PI3K-Akt Signaling, RTK Signaling, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Warburg Effect