SEC31A antibody (N-Term)

Catalog No. ABIN2856188

Product Details anti-SEC31A Antibody

Target: SEC31A (SEC31 Homolog A (SEC31A))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC31A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus), Zebrafish (Danio rerio), Cow (Bovine)

Cross-Reactivity (Details): Mouse (98 %), Chicken (93 %), Rat (97 %), Zebrafish (81 %), Bovine (97 %)

Characteristics: Rabbit Polyclonal antibody to SEC31A (SEC31 homolog A (S. cerevisiae))
SEC31A antibody [N1N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human SEC31A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A549 , HeLa , Jurkat , NT2D1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.7 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for SEC31A

Target: SEC31A (SEC31 Homolog A (SEC31A))

Alternative Name: SEC31A (SEC31A Products)

Synonyms: ABP125 antibody, ABP130 antibody, HSPC334 antibody, SEC31L1 antibody, Sec31l1 antibody, VAP1 antibody, 1810024J13Rik antibody, AU042160 antibody, HSPC275 antibody, cb354 antibody, sec31p antibody, zgc:63547 antibody, SEC31 homolog A, COPII coat complex component antibody, Sec31 homolog A (S. cerevisiae) antibody, SEC31A antibody, Sec31a antibody, sec31a antibody

Background: The protein encoded by this gene is similar to yeast Sec31 protein. Yeast Sec31 protein is known to be a component of the COPII protein complex which is responsible for vesicle budding from endoplasmic reticulum (ER). This protein was found to colocalize with SEC13, one of the other components of COPII , in the subcellular structures corresponding to the vesicle transport function. An immunodepletion experiment confirmed that this protein is required for ER-Golgi transport. Alternative splicing results in multiple transcript variants encoding different isoforms.

Molecular Weight: 133 kDa

Gene ID: 22872

Pathways: ER-Nucleus Signaling