ZEB1 antibody

Catalog No. ABIN2856075

Product Details anti-ZEB1 Antibody

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to ZEB1 (zinc finger E-box binding homeobox 1)
ZEB1 antibody [N2C1], Internal

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human ZEB1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , HeLa , HepG2 , Neuro2A , NIH-3T3 , C2C12

Validation: Comparison, KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ZEB1 antibody (ABIN2856075)

Fang, Lin, Liang, Liang: "A novel c-Kit/phospho-prohibitin axis enhances ovarian cancer stemness and chemoresistance via Notch3-PBX1 and β-catenin-ABCG2 signaling." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 42, (2020) (PubMed).

Huang, Wang, Lo, Tseng, Chen, Wu, Hou, Yuan: "Visfatin Mediates Malignant Behaviors through Adipose-Derived Stem Cells Intermediary in Breast Cancer." in: Cancers, Vol. 12, Issue 1, (2019) (PubMed).

Yoriki, Mori, Kokabu, Matsushima, Umemura, Tarumi, Kitawaki: "Estrogen-related receptor alpha induces epithelial-mesenchymal transition through cancer-stromal interactions in endometrial cancer." in: Scientific reports, Vol. 9, Issue 1, pp. 6697, (2019) (PubMed).

Yin, Yin, Wang, Zhou, Liu, Yang, Wang, Lu: "MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells." in: Oncotarget, Vol. 7, Issue 17, pp. 24537-48, (2017) (PubMed).

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: zinc finger E-box binding homeobox 1 (ZEB1 Products)

Background: ZEB1 encodes a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene (MIM 147680) expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site (Williams et al., 1991 [PubMed 1840704]).[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 124 kDa

Gene ID: 6935

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation