PCCB antibody (Center)

Catalog No. ABIN2856061

Product Details anti-PCCB Antibody

Target: PCCB (Propionyl CoA Carboxylase beta Polypeptide (PCCB))

Binding Specificity: Center

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCB antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Cow (Bovine), Pig (Porcine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)

Cross-Reactivity (Details): Pig (94 %), Rat (91 %), Xenopus laevis (90 %), Zebrafish (90 %), Bovine (97 %)

Characteristics: Rabbit Polyclonal antibody to PCCB (propionyl Coenzyme A carboxylase, beta polypeptide)
PCCB antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PCCB. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HepG2

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.54 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PCCB

Target: PCCB (Propionyl CoA Carboxylase beta Polypeptide (PCCB))

Alternative Name: PCCB (PCCB Products)

Synonyms: 1300012P06Rik antibody, AI314687 antibody, R74805 antibody, propionyl-CoA carboxylase beta subunit antibody, propionyl Coenzyme A carboxylase, beta polypeptide antibody, PCCB antibody, Pccb antibody

Background: The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2).

Cellular Localization: Mitochondrion matrix

Molecular Weight: 58 kDa

Gene ID: 5096

Pathways: Monocarboxylic Acid Catabolic Process