POU2F1 antibody

Catalog No. ABIN2856060

Product Details anti-POU2F1 Antibody

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POU2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human, Rat

Characteristics: Rabbit Polyclonal antibody to OCT1 (POU domain, class 2, transcription factor 1)
OCT1 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human OCT1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T , A431 , HeLa , HepG2

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.49 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-POU2F1 antibody (ABIN2856060)

Kitano, Kino, Yamamoto, Takitani, Miyoshi, Ishida, Saito, Arima, Satoh: "Bioinformatics Data Mining Approach Suggests Coexpression of AGTPBP1 with an ALS-linked Gene C9orf72." in: Journal of central nervous system disease, Vol. 7, pp. 15-26, (2015) (PubMed).

Target Details for POU2F1

Target: POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))

Alternative Name: POU class 2 homeobox 1 (POU2F1 Products)

Background: The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM]

Cellular Localization: Nucleus

Molecular Weight: 76 kDa

Gene ID: 5451

UniProt: P14859