ETFA antibody (Center)

Catalog No. ABIN2856049

Product Details anti-ETFA Antibody

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ETFA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Characteristics: Rabbit Polyclonal antibody to ETFA (electron-transfer-flavoprotein, alpha polypeptide)
ETFA antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human ETFA. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: A431 , HeLa , PC-12

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ETFA

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Alternative Name: ETFA (ETFA Products)

Synonyms: 2010200I21Rik antibody, D9Ertd394e antibody, ETF antibody, EMA antibody, GA2 antibody, MADD antibody, cb1020 antibody, fd06h11 antibody, wu:fd06h11 antibody, ema antibody, ga2 antibody, madd antibody, electron transfer flavoprotein alpha subunit antibody, electron-transfer-flavoprotein, alpha polypeptide antibody, putative electron-transfer-flavoprotein,alpha polypeptide antibody, electron transfer flavoprotein subunit alpha, mitochondrial antibody, electron transferring flavoprotein, alpha polypeptide antibody, electron transfer flavoprotein alpha subunit L homeolog antibody, ETFA antibody, Tc00.1047053503559.109 antibody, Tc00.1047053511693.90 antibody, LMJF_28_1140 antibody, LOC100194695 antibody, etfa antibody, Etfa antibody, etfa.L antibody

Background: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Mitochondrion matrix

Molecular Weight: 35 kDa

Gene ID: 2108

Pathways: SARS-CoV-2 Protein Interactome