LOXL2 antibody

Catalog No. ABIN2856034

Product Details anti-LOXL2 Antibody

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LOXL2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Specificity: Mouse reactivity has been validated by IHC-P only, not yet been tested by other applications.

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to LOXL2 (lysyl oxidase-like 2)
LOXL2 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human LOXL2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG , SK-N-SH , SK-N-AS , 293T transfected with LOXL2

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.86 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7), 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LOXL2 antibody (ABIN2856034)

Cao, Lin, Zhi, Lazare, Meng, Wu, Gao, Wei, Wu: "LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype." in: Frontiers in oncology, Vol. 10, pp. 284, (2020) (PubMed).

Mahjour, Dambal, Shrestha, Singh, Noonan, Kantarci, Trackman: "Mechanism for oral tumor cell lysyl oxidase like-2 in cancer development: synergy with PDGF-AB." in: Oncogenesis, Vol. 8, Issue 5, pp. 34, (2019) (PubMed).

Wang, Zhao, Zhu, Liu, Liang, Liu, Zhang, Dong, Sun: "HIF-1α promoted vasculogenic mimicry formation in hepatocellular carcinoma through LOXL2 up-regulation in hypoxic tumor microenvironment." in: Journal of experimental & clinical cancer research : CR, Vol. 36, Issue 1, pp. 60, (2018) (PubMed).

Aumiller, Strobel, Romeike, Schuler, Stierstorfer, Kreuz: "Comparative analysis of lysyl oxidase (like) family members in pulmonary fibrosis." in: Scientific reports, Vol. 7, Issue 1, pp. 149, (2018) (PubMed).

Bais, Kukuruzinska, Trackman: "Orthotopic non-metastatic and metastatic oral cancer mouse models." in: Oral oncology, Vol. 51, Issue 5, pp. 476-82, (2015) (PubMed).

Target Details for LOXL2

Target: LOXL2 (Lysyl Oxidase-Like 2 (LOXL2))

Alternative Name: lysyl oxidase like 2 (LOXL2 Products)

Synonyms: LOR2 antibody, WS9-14 antibody, CG4402 antibody, Dmel\\CG4402 antibody, Dmloxl-2 antibody, dmlox-2 antibody, 1110004B06Rik antibody, 4930526G11Rik antibody, 9430067E15Rik antibody, GB13360 antibody, lox2-like antibody, LOXL2 antibody, lor2 antibody, loxl-2 antibody, ws9-14 antibody, im:7136137 antibody, si:dkeyp-32b1.1 antibody, wu:fk12g04 antibody, zgc:158414 antibody, lysyl oxidase like 2 antibody, lysyl oxidase-like 2 antibody, lysyl oxidase homolog 4 antibody, lysyl oxidase like 2 L homeolog antibody, lysyl oxidase-like 2b antibody, LOXL2 antibody, lox2 antibody, Loxl2 antibody, LOC408544 antibody, loxl2.L antibody, loxl2 antibody, loxl2b antibody

Background: This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

Cellular Localization: Secreted , extracellular space

Molecular Weight: 87 kDa

Gene ID: 4017

UniProt: Q9Y4K0

Pathways: Chromatin Binding