PSMD14 antibody (Center)

Catalog No. ABIN2856031

This Rabbit Polyclonal antibody specifically detects PSMD14 in WB, IF, IP, ICC and IHC (p). It exhibits reactivity toward Humanand has been mentioned in 1 publication.

Quick Overview for PSMD14 antibody (Center) (ABIN2856031)

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PSMD14 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PSMD14 Antibody

Binding Specificity: Center

Characteristics: Rabbit Polyclonal antibody to MAD1 (MAD1 mitotic arrest deficient-like 1 (yeast))
MAD1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human MAD1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , HeLa , HepG2 , A375

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PSMD14 antibody (ABIN2856031)

Alfonso-Pérez, Hayward, Holder, Gruneberg, Barr: "MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling." in: The Journal of cell biology, Vol. 218, Issue 4, pp. 1108-1117, (2020) (PubMed).

Target Details for PSMD14

Target: PSMD14 (Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 14 (PSMD14))

Alternative Name: MAD1

Background: MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene.

Cellular Localization: Nucleus , Kinetochore

Molecular Weight: 83 kDa

Gene ID: 8379

Pathways: Mitotic G1-G1/S Phases, DNA Replication, M Phase, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Ubiquitin Proteasome Pathway