Dystroglycan antibody

Catalog No. ABIN2856020

Product Details anti-Dystroglycan Antibody

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Dystroglycan antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Cross-Reactivity: Horse, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Dystroglycan (dystroglycan 1 (dystrophin-associated glycoprotein 1))
DAG1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DAG1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: U87-MG membrane fraction extract , MCF-7 membrane extracts , 293T membrane extracts

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.21 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Dystroglycan antibody (ABIN2856020)

Jae, Raaben, Herbert, Kuehne, Wirchnianski, Soh, Stubbs, Janssen, Damme, Saftig, Whelan, Dye, Brummelkamp: "Virus entry. Lassa virus entry requires a trigger-induced receptor switch." in: Science (New York, N.Y.), Vol. 344, Issue 6191, pp. 1506-10, (2014) (PubMed).

Target Details for Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Alternative Name: dystroglycan 1 (DAG1 Products)

Background: Dystroglycan is a laminin binding component of the dystrophin-glycoprotein complex which provides a linkage between the subsarcolemmal cytoskeleton and the extracellular matrix. Dystroglycan 1 is a candidate gene for the site of the mutation in autosomal recessive muscular dystrophies. The dramatic reduction of dystroglycan 1 in Duchenne muscular dystrophy leads to a loss of linkage between the sarcolemma and extracellular matrix, rendering muscle fibers more susceptible to necrosis. Dystroglycan also functions as dual receptor for agrin and laminin-2 in the Schwann cell membrane. The muscle and nonmuscle isoforms of dystroglycan differ by carbohydrate moieties but not protein sequence.

Cellular Localization: Alpha-dystroglycan: Secreted , extracellular space , Beta-dystroglycan: Cell membrane , Cytoplasm , cytoskeleton

Molecular Weight: 97 kDa

Gene ID: 1605

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus