Proteasome 20S alpha 4 (Center) antibody

Catalog No. ABIN2855988

This anti- antibody is a Rabbit Polyclonal antibody detecting in ICC, IF and WB. Suitable for Human.

Quick Overview for Proteasome 20S alpha 4 (Center) antibody (ABIN2855988)

Target: Proteasome 20S alpha 4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)

Product Details

Binding Specificity: Center

Cross-Reactivity: Pig (Porcine), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to Proteasome 20S alpha 4 (proteasome (prosome, macropain) subunit, alpha type, 4)
Proteasome 20S alpha 4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details

Target: Proteasome 20S alpha 4

Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 29 kDa

Gene ID: 5685