Proteasome 20S alpha 4 (Center) antibody
Reactivity: Human
ICC, IF, WB
Host: Rabbit
Polyclonal
unconjugated
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- Target
- Proteasome 20S alpha 4
- Binding Specificity
- Center
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
- Cross-Reactivity
- Pig (Porcine), Rhesus Monkey, Cow (Bovine)
- Cross-Reactivity (Details)
- Pig (100 %), Rhesus Monkey (100 %), Bovine (100 %)
- Characteristics
-
Rabbit Polyclonal antibody to Proteasome 20S alpha 4 (proteasome (prosome, macropain) subunit, alpha type, 4)
Proteasome 20S alpha 4 antibody - Purification
- Purified by antigen-affinity chromatography.
- Immunogen
- Recombinant protein encompassing a sequence within the center region of human Proteasome 20S alpha 4. The exact sequence is proprietary.
- Isotype
- IgG
-
- Application Notes
- Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*
- Comment
-
Positive Control: HeLa
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
-
- Target
- Proteasome 20S alpha 4
- Background
-
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Cellular Localization: Cytoplasm , Nucleus - Molecular Weight
- 29 kDa
- Gene ID
- 5685
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