TPI1 antibody (C-Term)

Catalog No. ABIN2855867

Product Details anti-TPI1 Antibody

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Triosephosphate isomerase (triosephosphate isomerase 1)
Triosephosphate isomerase antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Triosephosphate isomerase. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.27 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TPI1 antibody (ABIN2855867)

Chen, Lin, Chen, Cheng, Cheng, Imai et al.: "Effect of prednisolone on glyoxalase 1 in an inbred mouse model of aristolochic acid nephropathy using a proteomics method with fluorogenic derivatization-liquid chromatography-tandem mass ..." in: PLoS ONE, Vol. 15, Issue 1, pp. e0227838, (2020) (PubMed).

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: triosephosphate isomerase 1 (TPI1 Products)

Synonyms: TIM antibody, TPI antibody, TIM1 antibody, hTIM antibody, tpi antibody, tpi1 antibody, TPI1 antibody, AI255506 antibody, Tpi antibody, Tpi-1 antibody, GB17473 antibody, Tim antibody, CG2171 antibody, Dmel\\CG2171 antibody, ND14 antibody, TPIS antibody, sgk antibody, wstd antibody, DrTPI-A antibody, DrTPI-B antibody, TIM-B antibody, cb179 antibody, wu:fb51c02 antibody, triosephosphate isomerase 1 antibody, timeless circadian regulator antibody, triosephosphate isomerase 1 S homeolog antibody, triosephosphate isomerase antibody, triose-phosphate isomerase antibody, Triose-phosphate isomerase antibody, triose-phosphate isomerase, putative antibody, timeless circadian clock antibody, Triose phosphate isomerase antibody, Triosephosphate isomerase antibody, triosephosphate isomerase 1a antibody, triosephosphate isomerase 1b antibody, TPI1 antibody, TIMELESS antibody, tpi1 antibody, Tpi1 antibody, tpi1.S antibody, Tpi antibody, tpiA antibody, CNE05000 antibody, Fnod_0738 antibody, Tlet_0996 antibody, SARE_RS16215 antibody, PKH_126640 antibody, Timeless antibody, tpi-1 antibody, tpi1a antibody, tpi1b antibody

Background: This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.

Molecular Weight: 31 kDa

Gene ID: 7167

UniProt: P60174

Pathways: Cell RedoxHomeostasis