GPC1 antibody

Catalog No. ABIN2855849

Product Details anti-GPC1 Antibody

Target: GPC1 (Glypican 1 (GPC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPC1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Glypican 1 (glypican 1)
Glypican 1 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Glypican 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. FACS: 1:50-1:200. IP: 1:100-1:500. ELISA: 1:1000-1:10000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: MDA-MB-231 , MDA-MB-231 (24 μg/ml Tunicamycin for 16hr) , U87-MG membrane extracts , U87-MG conditioned medium , Mouse brain , mouse fetal brain , A431 , MCF-7 , AsPC-1

Validation: Comparison, KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.58 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GPC1 antibody (ABIN2855849)

Lu, Niu, Liu, Gao, Sun, Zhao: "Elevated glypican-1 expression is associated with an unfavorable prognosis in pancreatic ductal adenocarcinoma." in: Cancer medicine, Vol. 6, Issue 6, pp. 1181-1191, (2018) (PubMed).

Huang, Ge, Izzi, Greenspan: "α3 Chains of type V collagen regulate breast tumour growth via glypican-1." in: Nature communications, Vol. 8, pp. 14351, (2018) (PubMed).

Target Details for GPC1

Target: GPC1 (Glypican 1 (GPC1))

Alternative Name: glypican 1 (GPC1 Products)

Synonyms: gpc1 antibody, zgc:63947 antibody, GPC1 antibody, Glypican-1 antibody, glypican antibody, AI462976 antibody, HSPG M12 antibody, HSPGM12 antibody, glypican 1b antibody, glypican 1 antibody, gpc1b antibody, GPC1 antibody, gpc1 antibody, Gpc1 antibody

Background: Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Molecular Weight: 62 kDa

Gene ID: 2817

UniProt: P35052

Pathways: Glycosaminoglycan Metabolic Process, Regulation of Muscle Cell Differentiation