GOLGA5 antibody

Catalog No. ABIN2855796

Product Details anti-GOLGA5 Antibody

Target: GOLGA5 (Golgin A5 (GOLGA5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOLGA5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Hamster, Human, Mouse

Characteristics: Rabbit Polyclonal antibody to GOLGA5 (golgin A5)
GOLGA5 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human GOLGA5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , A549

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-GOLGA5 antibody (ABIN2855796)

Pérez-Rodriguez, de Jesús Ramírez-Lira, Wulff, Voldbor, Ramírez, Trujillo-Roldán, Valdez-Cruz: "Enrichment of microsomes from Chinese hamster ovary cells by subcellular fractionation for its use in proteomic analysis." in: PLoS ONE, Vol. 15, Issue 8, pp. e0237930, (2020) (PubMed).

Popa, Villeneuve, Stewart, Perez Garcia, Petrunkina Harrison, Moreau: "Genome-wide CRISPR screening identifies new regulators of glycoprotein secretion." in: Wellcome open research, Vol. 4, pp. 119, (2019) (PubMed).

Chang, Chen, Shen, Kung, Chuang, Chen: "Selective serotonin reuptake inhibitor, fluoxetine, impairs E-cadherin-mediated cell adhesion and alters calcium homeostasis in pancreatic beta cells." in: Scientific reports, Vol. 7, Issue 1, pp. 3515, (2019) (PubMed).

Reiling, Olive, Sanyal, Carette, Brummelkamp, Ploegh, Starnbach, Sabatini: "A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens." in: Nature cell biology, Vol. 15, Issue 12, pp. 1473-85, (2013) (PubMed).

Target Details for GOLGA5

Target: GOLGA5 (Golgin A5 (GOLGA5))

Alternative Name: golgin A5 (GOLGA5 Products)

Synonyms: GOLIM5 antibody, RFG5 antibody, ret-II antibody, golim5 antibody, ret-ii antibody, rfg5 antibody, Ret-II antibody, im:7153094 antibody, sb:cb898 antibody, wu:fd50h11 antibody, zgc:66400 antibody, golgin A5 antibody, golgin A5 L homeolog antibody, golgi autoantigen, golgin subfamily a, 5 antibody, GOLGA5 antibody, golga5.L antibody, golga5 antibody, Golga5 antibody

Background: The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes a member of the golgin family of proteins, whose members localize to the Golgi. This protein is a coiled-coil membrane protein that has been postulated to play a role in vesicle tethering and docking. Translocations involving this gene and the ret proto-oncogene have been found in tumor tissues, the chimeric sequences have been designated RET-II and PTC5.

Cellular Localization: Golgi apparatus membrane, Single-pass type IV membrane protein

Molecular Weight: 83 kDa

Gene ID: 9950

UniProt: Q8TBA6