KRT14 antibody

Catalog No. ABIN2855778

Product Details anti-KRT14 Antibody

Target: KRT14 (Keratin 14 (KRT14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT14 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Dog, Human, Rat

Characteristics: Rabbit Polyclonal antibody to Cytokeratin 14 (keratin 14)
Cytokeratin 14 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 14. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KRT14 antibody (ABIN2855778)

Kim, Ohn, Yoon, Kang, Park, Kim, Lee, Hwang, Kim, Kim, Kwon: "Priming mobilization of hair follicle stem cells triggers permanent loss of regeneration after alkylating chemotherapy." in: Nature communications, Vol. 10, Issue 1, pp. 3694, (2019) (PubMed).

Target Details for KRT14

Target: KRT14 (Keratin 14 (KRT14))

Alternative Name: keratin 14 (KRT14 Products)

Synonyms: CK14 antibody, EBS3 antibody, EBS4 antibody, K14 antibody, NFJ antibody, AI626930 antibody, Krt-1.14 antibody, Krt1-14 antibody, Ka14 antibody, ouro2 antibody, keratin 14 antibody, keratin 14, type I L homeolog antibody, KRT14 antibody, Krt14 antibody, krt14.L antibody

Background: This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 Molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 52 kDa

Gene ID: 3861

UniProt: P02533