EIF4E2 antibody

Catalog No. ABIN2855740

Product Details anti-EIF4E2 Antibody

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to EIF4EL3 (eukaryotic translation initiation factor 4E family member 2)
EIF4E2 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human EIF4E2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.27 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-EIF4E2 antibody (ABIN2855740)

Juszkiewicz, Slodkowicz, Lin, Freire-Pritchett, Peak-Chew, Hegde: "Ribosome collisions trigger cis-acting feedback inhibition of translation initiation." in: eLife, Vol. 9, (2020) (PubMed).

Garzia, Jafarnejad, Meyer, Chapat, Gogakos, Morozov, Amiri, Shapiro, Molina, Tuschl, Sonenberg: "The E3 ubiquitin ligase and RNA-binding protein ZNF598 orchestrates ribosome quality control of premature polyadenylated mRNAs." in: Nature communications, Vol. 8, pp. 16056, (2018) (PubMed).

Target Details for EIF4E2

Target: EIF4E2 (Eukaryotic Translation Initiation Factor 4E Family Member 2 (EIF4E2))

Alternative Name: eukaryotic translation initiation factor 4E family member 2 (EIF4E2 Products)

Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Molecular Weight: 28 kDa

Gene ID: 9470

UniProt: O60573

Pathways: SARS-CoV-2 Protein Interactome