CD55 antibody (Center)

Catalog No. ABIN2855732

Product Details anti-CD55 Antibody

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD55 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: Rabbit Polyclonal antibody to CD55 (CD55 Molecule, decay accelerating factor for complement (Cromer blood group))
CD55 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human CD55. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: K562 , THP-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CD55 antibody (ABIN2855732)

Kwon, Kim, Hwang, Kim, Kim, Kim, Lee, Im, Lee, Hwang: "Generation of α-1,3-galactosyltransferase knocked-out transgenic cloned pigs with knocked-in five human genes." in: Transgenic research, Vol. 26, Issue 1, pp. 153-163, (2018) (PubMed).

Target Details for CD55

Target: CD55 (Complement Decay-Accelerating Factor (CD55))

Alternative Name: CD55 (CD55 Products)

Synonyms: CR antibody, CROM antibody, DAF antibody, TC antibody, Daf antibody, Daf-GPI antibody, Daf1 antibody, GPI-DAF antibody, CD55 molecule (Cromer blood group) antibody, CD55 molecule, decay accelerating factor for complement antibody, CD55 antibody, Cd55 antibody

Background: This gene encodes a protein involved in the regulation of the complement cascade. The encoded glycoprotein is also known as the decay-accelerating factor (DAF), binding of DAF to complement proteins accelerates their decay, disrupting the cascade and preventing damage to host cells. Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM). Two alternatively spliced transcripts encoding different proteins have been identified. The predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. Additional, alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Cellular Localization: Isoform 1: Membrane , Isoform 2: Cell membrane , GPI-anchor

Molecular Weight: 41 kDa

Gene ID: 1604

Pathways: Complement System, Regulation of Leukocyte Mediated Immunity