NOX1 antibody

Catalog No. ABIN2855720

Product Details anti-NOX1 Antibody

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOX1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to NOX1 (NADPH oxidase 1)
NOX1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NOX1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: JC , BCL-1 , 293T , A431 , HeLa

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.19 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NOX1 antibody (ABIN2855720)

Liang, Ma, Zhong, Dhar, Liu, Xu, Koyama, Nishio, Karin, Karin, Mccubbin, Zhang, Hu, Yang, Chen, Ganguly, Lan, Karin, Kisseleva, Brenner: "NADPH Oxidase 1 in Liver Macrophages Promotes Inflammation and Tumor Development in Mice." in: Gastroenterology, Vol. 156, Issue 4, pp. 1156-1172.e6, (2019) (PubMed).

Sandrini, Ieraci, Amadio, Popoli, Tremoli, Barbieri: "Apocynin Prevents Abnormal Megakaryopoiesis and Platelet Activation Induced by Chronic Stress." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 9258937, (2018) (PubMed).

Target Details for NOX1

Target: NOX1 (NADPH Oxidase 1 (NOX1))

Alternative Name: NADPH oxidase 1 (NOX1 Products)

Synonyms: GP91-2 antibody, MOX1 antibody, NOH-1 antibody, NOH1 antibody, NOX1a antibody, NOX1alpha antibody, Nox-1 antibody, Nox1 antibody, NADPH oxidase 1 antibody, NOX1 antibody, Nox1 antibody, nox1 antibody

Background: Voltage-gated proton (hydrogen) channels play an important role in cellular defense against acidic stress. They are unique among ion channels with respect to their extremely high selectivity, marked temperature dependence, and unitary conductance, which is 3 orders of magnitude lower than that of most other ion channels. NOX1 is a homolog of the catalytic subunit of the superoxide-generating NADPH oxidase of phagocytes, gp91phox. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Membrane, Multi-pass membrane protein

Molecular Weight: 65 kDa

Gene ID: 27035

UniProt: Q9Y5S8

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, Proton Transport