PAK1 antibody (Center)

Catalog No. ABIN2855705

Product Details anti-PAK1 Antibody

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Binding Specificity: Center

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Cow (Bovine)

Cross-Reactivity (Details): Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to PAK1 (p21 protein (Cdc42/Rac)-activated kinase 1)
PAK1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human PAK1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:10000*

Comment:

Positive Control: A431 , HeLa , BCL-1 , PC-12 , SH-SY5Y

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-PAK1 antibody (ABIN2855705)

Cai, Zhang, Zhang, Chen, Hu: "Effect of Th9/IL-9 on the growth of gastric cancer in nude mice." in: OncoTargets and therapy, Vol. 12, pp. 2225-2234, (2019) (PubMed).

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1 (PAK1 Products)

Synonyms: X-PAK1 antibody, XPak1 antibody, pak antibody, pak-1 antibody, pakalpha antibody, PAKalpha antibody, AW045634 antibody, PAK-1 antibody, Paka antibody, p21 protein (Cdc42/Rac)-activated kinase 1 S homeolog antibody, p21 (RAC1) activated kinase 1 antibody, p21 protein (Cdc42/Rac)-activated kinase 1 antibody, Serine/threonine-protein kinase pak-1 antibody, pak1.S antibody, PAK1 antibody, Pak1 antibody, pak-1 antibody

Background: PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , Cell junction , focal adhesion

Molecular Weight: 61 kDa

Gene ID: 5058

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis