DNAJB12 antibody (Center)

Catalog No. ABIN2855671

Product Details anti-DNAJB12 Antibody

Target: DNAJB12 (DnaJ (Hsp40) Homolog, Subfamily B, Member 12 (DNAJB12))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJB12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse (Murine), Chicken, Rat (Rattus), Xenopus laevis, Cow (Bovine), Xenopus tropicalis

Cross-Reactivity (Details): Mouse (96 %), Chicken (85 %), Rat (96 %), Xenopus laevis (80 %), Bovine (94 %), Xenopus tropicalis (82 %)

Characteristics: Rabbit Polyclonal antibody to DNAJB12 (DnaJ (Hsp40) homolog, subfamily B, member 12)
DNAJB12 antibody [N2C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human DNAJB12. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceIHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: A549 , H1299 , HCT116 , MCF-7

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.89 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for DNAJB12

Target: DNAJB12 (DnaJ (Hsp40) Homolog, Subfamily B, Member 12 (DNAJB12))

Alternative Name: DNAJB12 (DNAJB12 Products)

Background: DNAJB12 belongs to the evolutionarily conserved DNAJ/HSP40 family of proteins, which regulate molecular chaperone activity by stimulating ATPase activity. DNAJ proteins may have up to 3 distinct domains: a conserved 70-amino acid J domain, usually at the N terminus, a glycine/phenylalanine (G/F)-rich region, and a cysteine-rich domain containing 4 motifs resembling a zinc finger domain (Ohtsuka and Hata, 2000 [PubMed 11147971]).[supplied by OMIM]

Cellular Localization: Membrane

Molecular Weight: 42 kDa

Gene ID: 54788