UAP1 antibody

Catalog No. ABIN2855661

Product Details anti-UAP1 Antibody

Target: UAP1 (UDP-N-Acteylglucosamine Pyrophosphorylase 1 (UAP1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to UAP1 (UDP-N-acteylglucosamine pyrophosphorylase 1)
UAP1 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human UAP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-UAP1 antibody (ABIN2855661)

Andres, Blong, Evans, Rumachik, Yamaguchi, Pham, Thompson, Kohler, Bertozzi: "Chemical Modulation of Protein O-GlcNAcylation via OGT Inhibition Promotes Human Neural Cell Differentiation." in: ACS chemical biology, Vol. 12, Issue 8, pp. 2030-2039, (2018) (PubMed).

Target Details for UAP1

Target: UAP1 (UDP-N-Acteylglucosamine Pyrophosphorylase 1 (UAP1))

Alternative Name: UDP-N-acetylglucosamine pyrophosphorylase 1 (UAP1 Products)

Synonyms: fb63d12 antibody, fb64d12 antibody, zgc:55280 antibody, wu:fb63d12 antibody, wu:fb64d12 antibody, UAP1 antibody, agx antibody, agx1 antibody, agx2 antibody, spag2 antibody, AA420407 antibody, AA437972 antibody, AGX-1 antibody, AGX-2 antibody, AGX1 antibody, AgX antibody, ESTM38 antibody, SPAG2 antibody, AGX antibody, AGX2 antibody, RGD1561967 antibody, UDP-N-acetylglucosamine pyrophosphorylase 1 antibody, UDP-N-acetylglucosamine pyrophosphorylase 1 S homeolog antibody, UDP-N-acteylglucosamine pyrophosphorylase 1 antibody, UDP-N-acetylglucosamine pyrophosphorylase 1 L homeolog antibody, uap1 antibody, uap1.S antibody, UAP1 antibody, EUBREC_2612 antibody, uap1.L antibody, Uap1 antibody

Background: Converts UDP and GlcNAc-1-P into UDP-GlcNAc, and UDP and GalNAc-1-P into UDP-GalNAc. Isoform AGX1 has 2 to 3 times higher activity towards GalNAc-1-P, while isoform AGX2 has 8 times more activity towards GlcNAc-1-P.

Cellular Localization: Cytoplasm

Molecular Weight: 59 kDa

Gene ID: 6675

UniProt: Q16222