NRF2 antibody (Internal Region)

Catalog No. ABIN2855612

Product Details anti-NRF2 Antibody

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Binding Specificity: Internal Region

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NRF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP)

Characteristics: Rabbit Polyclonal antibody to NRF2 (nuclear factor (erythroid-derived 2)-like 2)
NRF2 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

Comment:

Positive Control: MDA-MB-231 NE , MDA-MB-231 NE treated tBHQ , 3xFlag-human NFE2L2-transfected 293T

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.51 mg/mL

Buffer: 1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NRF2 antibody (ABIN2855612)

Tsai, Chen, Lin, Chou, Chen, Ho, Lin, Hwang: "Miconazole Contributes to NRF2 Activation by Noncanonical P62-KEAP1 Pathway in Bladder Cancer Cells." in: Drug design, development and therapy, Vol. 14, pp. 1209-1218, (2020) (PubMed).

Batubara, Astuti, Prastya, Ilmiawati, Maeda, Suzuki, Hamamoto, Takemori: "The Antiaging Effect of Active Fractions and Ent-11α-Hydroxy-15-Oxo-Kaur-16-En-19-Oic Acid Isolated from Adenostemma lavenia (L.) O. Kuntze at the Cellular Level." in: Antioxidants (Basel, Switzerland), Vol. 9, Issue 8, (2020) (PubMed).

Chou, Lin, Dzhagalov, Chen, Lin, Lin, Chen, Chen, Fu: "Vaccine adjuvant activity of a TLR4-activating synthetic glycolipid by promoting autophagy." in: Scientific reports, Vol. 10, Issue 1, pp. 8422, (2020) (PubMed).

Neitzel, Seiwert, Göder, Diehl, Weber, Nagel, Stroh, Rasenberger, Christmann, Fahrer: "Lipoic Acid Synergizes with Antineoplastic Drugs in Colorectal Cancer by Targeting p53 for Proteasomal Degradation." in: Cells, Vol. 8, Issue 8, (2020) (PubMed).

Luo, Shang, Brooks, Jiagge, Zhu, Buschhaus, Conley, Fath, Davis, Gheordunescu, Wang, Harouaka, Lozier, Triner, McDermott, Merajver, Luker, Spitz, Wicha: "Targeting Breast Cancer Stem Cell State Equilibrium through Modulation of Redox Signaling." in: Cell metabolism, Vol. 28, Issue 1, pp. 69-86.e6, (2019) (PubMed).

García Trejo, Arellano Buendía, Sánchez Reyes, García Arroyo, Arguello García, Loredo Mendoza, Tapia, Sánchez Lozada, Osorio Alonso: "The Beneficial Effects of Allicin in Chronic Kidney Disease Are Comparable to Losartan." in: International journal of molecular sciences, Vol. 18, Issue 9, (2018) (PubMed).

Fragoulis, Siegl, Fendt, Jansen, Soppa, Brandenburg, Pufe, Weis, Wruck: "Oral administration of methysticin improves cognitive deficits in a mouse model of Alzheimer's disease." in: Redox biology, Vol. 12, pp. 843-853, (2018) (PubMed).

García-Trejo, Arellano-Buendía, Argüello-García, Loredo-Mendoza, García-Arroyo, Arellano-Mendoza, Castillo-Hernández, Guevara-Balcázar, Tapia, Sánchez-Lozada, Osorio-Alonso: "Effects of Allicin on Hypertension and Cardiac Function in Chronic Kidney Disease." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 3850402, (2017) (PubMed).

Arellano-Buendía, Tostado-González, García-Arroyo, Cristóbal-García, Loredo-Mendoza, Tapia, Sánchez-Lozada, Osorio-Alonso: "Anti-Inflammatory Therapy Modulates Nrf2-Keap1 in Kidney from Rats with Diabetes." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 4693801, (2017) (PubMed).

Target Details for NRF2

Target: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Alternative Name: NRF2 (NFE2L2 Products)

Synonyms: Nrf2 antibody, nfe2l2 antibody, wu:fc15g09 antibody, wu:fj67e03 antibody, NF-E2R2 antibody, NRF2 antibody, AI194320 antibody, nuclear factor, erythroid 2-like 2a antibody, nuclear factor, erythroid 2 like 2 S homeolog antibody, nuclear factor, erythroid 2 like 2 antibody, nuclear factor, erythroid derived 2, like 2 antibody, nuclear factor, erythroid 2-like 2 antibody, nfe2l2a antibody, nfe2l2.S antibody, NFE2L2 antibody, Nfe2l2 antibody

Background: NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Cellular Localization: Cytoplasm , cytosol , Nucleus

Molecular Weight: 68 kDa110

Gene ID: 4780

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling