ACTR2 antibody

Catalog No. ABIN2855610

Product Details anti-ACTR2 Antibody

Target: ACTR2 (Actin-Related Protein 2 (ACTR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Drosophila melanogaster, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to Arp2 (ARP2 actin-related protein 2 homolog (yeast))
Arp2 antibody [N1C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Arp2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: H1299

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.41 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-ACTR2 antibody (ABIN2855610)

Graziano, Town, Sitarska, Nagy, Fošnarič, Penič, Iglič, Kralj-Iglič, Gov, Diz-Muñoz, Weiner: "Cell confinement reveals a branched-actin independent circuit for neutrophil polarity." in: PLoS biology, Vol. 17, Issue 10, pp. e3000457, (2020) (PubMed).

Target Details for ACTR2

Target: ACTR2 (Actin-Related Protein 2 (ACTR2))

Alternative Name: actin related protein 2 (ACTR2 Products)

Background: The specific function of this gene has not yet been determined, however, the protein it encodes is known to be a major constituent of the ARP2/3 complex. This complex is located at the cell surface and is essential to cell shape and motility through lamellipodial actin assembly and protrusion. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , cytoskeleton , Cell projection

Molecular Weight: 45 kDa

Gene ID: 10097

UniProt: P61160

Pathways: RTK Signaling, Regulation of Actin Filament Polymerization