Lamin B1 antibody

Catalog No. ABIN2855607

Product Details anti-Lamin B1 Antibody

Target: Lamin B1 (LMNB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Hamster, Human, Mouse

Characteristics: Rabbit Polyclonal antibody to Lamin B1 (lamin B1)
Lamin B1 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validatio: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Lamin B1 antibody (ABIN2855607)

Siew, Chen, Chen, Chen, Chen, Soong, Wu, Chang, Chan, Lin, Liu, Chern: "Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington's disease." in: Nature communications, Vol. 10, Issue 1, pp. 3473, (2019) (PubMed).

Silva, Pentz, Figueira, Arora, Lee, Hodson, Wischnewski, Deans, Azzalin: "FANCM limits ALT activity by restricting telomeric replication stress induced by deregulated BLM and R-loops." in: Nature communications, Vol. 10, Issue 1, pp. 2253, (2019) (PubMed).

Hsieh, Wang, Changou, Chin, Yang, Lai, Lee, Yang, Whang-Peng, Liu, Lin, Mousa, Davis: "Crosstalk between integrin αvβ3 and ERα contributes to thyroid hormone-induced proliferation of ovarian cancer cells." in: Oncotarget, Vol. 8, Issue 15, pp. 24237-24249, (2018) (PubMed).

Arora, Lee, Wischnewski, Brun, Schwarz, Azzalin: "RNaseH1 regulates TERRA-telomeric DNA hybrids and telomere maintenance in ALT tumour cells." in: Nature communications, Vol. 5, pp. 5220, (2014) (PubMed).

Target Details for Lamin B1

Target: Lamin B1 (LMNB1)

Alternative Name: lamin B1 (LMNB1 Products)

Synonyms: MGC52550 antibody, Lamin-L(I) antibody, lamin-b antibody, lmn antibody, lmn2 antibody, lmnb antibody, LMNB1 antibody, ADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, fc06g01 antibody, wu:fc06g01 antibody, lamin B1 L homeolog antibody, lamin B1 antibody, lmnb1.L antibody, lmnb1 antibody, LMNB1 antibody, Lmnb1 antibody

Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1.

Cellular Localization: Nucleus inner membrane, Lipid-anchor, Nucleoplasmic side

Molecular Weight: 66 kDa

Gene ID: 4001

UniProt: P20700

Pathways: Apoptosis, Caspase Cascade in Apoptosis