KLF5 antibody

Catalog No. ABIN2855604

Product Details anti-KLF5 Antibody

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KLF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to KLF5 (Kruppel-like factor 5 (intestinal))
KLF5 antibody

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: KLF5-transfected 293T , HeLa (500 μM CoCl2 treatment for 24 hr and 10 μM MG132 treatment for 2 hr)

Validation: KO/KD, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.8 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KLF5 antibody (ABIN2855604)

Sun, Li, Wang, Yue, Bai, Bian, Hao, Sun, Zhang, Liu: "Increased AT2R expression is induced by AT1R autoantibody via two axes, Klf-5/IRF-1 and circErbB4/miR-29a-5p, to promote VSMC migration." in: Cell death & disease, Vol. 11, Issue 6, pp. 432, (2020) (PubMed).

Ma, Chang, Zhao, Zhao, Xiong, Cao, Yuan, Zhang, Wang, Geng, Zheng, Li: "KLF5 promotes cervical cancer proliferation, migration and invasion in a manner partly dependent on TNFRSF11a expression." in: Scientific reports, Vol. 7, Issue 1, pp. 15683, (2019) (PubMed).

Target Details for KLF5

Target: KLF5 (Kruppel-Like Factor 5 (Intestinal) (KLF5))

Alternative Name: Kruppel like factor 5 (KLF5 Products)

Synonyms: KLF5 antibody, klf5 antibody, MGC115081 antibody, BTEB2 antibody, Bteb2 antibody, IKLF antibody, bteb2 antibody, 4930520J07Rik antibody, CKLF antibody, Kruppel like factor 5 antibody, Kruppel-like factor 5 (intestinal) S homeolog antibody, Kruppel-like factor 5 antibody, KLF5 antibody, klf5.S antibody, Klf5 antibody

Background: This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor.

Cellular Localization: Nucleus

Molecular Weight: 51 kDa

Gene ID: 688

UniProt: Q13887