Calpain 5 antibody

Catalog No. ABIN2855597

Product Details anti-Calpain 5 Antibody

Target: Calpain 5 (CAPN5)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calpain 5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to Calpain-5 (calpain 5)
Calpain-5 antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 5. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse uterus , RMS-13

Validation: Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.39 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Calpain 5 antibody (ABIN2855597)

Velez, Bassuk, Schaefer, Brooks, Gakhar, Mahajan, Kahn, Tsang, Ferguson, Mahajan: "A novel de novo CAPN5 mutation in a patient with inflammatory vitreoretinopathy, hearing loss, and developmental delay." in: Cold Spring Harbor molecular case studies, Vol. 4, Issue 3, (2019) (PubMed).

Schaefer, Toral, Velez, Cox, Baker, Borcherding, Colgan, Bondada, Mashburn, Yu, Geddes, Tsang, Bassuk, Mahajan: "Calpain-5 Expression in the Retina Localizes to Photoreceptor Synapses." in: Investigative ophthalmology & visual science, Vol. 57, Issue 6, pp. 2509-21, (2016) (PubMed).

Target Details for Calpain 5

Target: Calpain 5 (CAPN5)

Alternative Name: calpain 5 (CAPN5 Products)

Synonyms: HTRA3 antibody, nCL-3 antibody, Htra3 antibody, capn5 antibody, MGC53448 antibody, MGC89353 antibody, CAPN5 antibody, zgc:153446 antibody, calpain 5 antibody, calpain 5 L homeolog antibody, calpain 5a antibody, calpain 6 antibody, CAPN5 antibody, Capn5 antibody, capn5.L antibody, capn5 antibody, capn5a antibody, Bm1_30735 antibody, Bm1_30740 antibody, CAPN6 antibody, LOAG_10085 antibody

Background: Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3.

Molecular Weight: 73 kDa

Gene ID: 726

UniProt: O15484