ACTN1 antibody

Catalog No. ABIN2855589

Product Details anti-ACTN1 Antibody

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACTN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to alpha Actinin 1 (actinin, alpha 1)
alpha Actinin 1 antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Actinin 1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , 293T , A431 , HeLa , HepG2 , PC-12 , Rat2 , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.09 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ACTN1

Target: ACTN1 (Actinin, alpha 1 (ACTN1))

Alternative Name: actinin alpha 1 (ACTN1 Products)

Synonyms: ACTN1 antibody, actinin antibody, BDPLT15 antibody, 3110023F10Rik antibody, Actn1a antibody, actinin alpha 1 antibody, actinin, alpha 1 antibody, actinin alpha 1 L homeolog antibody, ACTN1 antibody, actn1 antibody, Actn1 antibody, actn1.L antibody

Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Cytoplasm , cytoskeleton , myofibril , sarcomere , Z-disk

Molecular Weight: 103 kDa

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization