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GRP94 antibody (Center)

Name: GRP94 antibody (Center)
Brand: antibodies-online
SKU: ABIN2855578
Price: 609.18 USD
Availability: InStock

HSP90B1 Reactivity: Human, Mouse WB, IF, IP, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

WB Image Sample (30 ug of whole cell lysate) A: H1299 B: Hela S3 7. (GRP94 antibody (Center))

IHC-P Image Immunohistochemical analysis of paraffin-embedded SCM-1 xenograft , using HSP90B, antibody at 1:500 dilution. (GRP94 antibody (Center))

IHC

WB Image Sample (30 ug of whole cell lysate) A: NIH-3T3 7. (GRP94 antibody (Center))

IP Image Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 μg of GRP94 antibody [N1N3], Western blot analysis was performed using GRP94 antibody [N1N3], EasyBlot anti-Rabbit IgG was used as a secondary reagent. (GRP94 antibody (Center))

ICC/IF Image GRP94 antibody [N1N3] detects GRP94 protein at cytoplasm by immunofluorescent analysis. (GRP94 antibody (Center))

ER proteostasis regulators induce ATF6 targets at the protein level and time-dependence of ATF6 and XBP1s target activation. (GRP94 antibody (Center))

Study of cellular SIL1 immunoreactivity and presence of ER-stress in Hek293 cells overexpressing SIL1 or its variant forms. (GRP94 antibody (Center))

7 images

(1 reference)

Catalog No. ABIN2855578

Datasheet as PDF

Target See all GRP94 (HSP90B1) Antibodies

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
Binding Specificity
19

18

16

7

7

7

7

6

6

4

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1
Center
Reactivity
136

95

61

39

35

31

31

30

28

28

26

26

25

17

10

2

2

1

1
Human, Mouse
Host
99

39

20

2

1
Rabbit
Compatible Secondaries
Clonality
101

60
Polyclonal
Conjugate
65

11

11

10

9

8

4

4

4

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GRP94 antibody is un-conjugated
Application
141

81

62

60

44

43

34

18

13

13

13

11

4

4

2

2

1
Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Rhesus Monkey, Chimpanzee

Cross-Reactivity (Details)

Rhesus Monkey (100 %), Chimpanzee (100 %)

Characteristics

Rabbit Polyclonal antibody to GRP94 (heat shock protein 90 kDa beta (Grp94), member 1)
GRP94 antibody [N1N3]

Purification

Purified by antigen-affinity chromatography.

Immunogen

Recombinant protein encompassing a sequence within the center region of human GRP94. The exact sequence is proprietary.

Isotype

IgG
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody
HSP90B1 Reactivity: Human, Mouse, Rat WB, IF, IP, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibody
Verified HSP90B1 Reactivity: Human, Mouse WB, IHC, FACS, IF, ELISA Host: Goat Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibody
HSP90B1 Reactivity: Human WB, IF, IHC (p), EIA Host: Mouse Monoclonal 2H3 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibody
HSP90B1 Reactivity: Human, Mouse, Rat WB, IHC, IF, ELISA, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 16-43), (N-Term) antibody
HSP90B1 Reactivity: Human, Mouse, Hamster WB, FACS, IF, IHC (p) Host: Rabbit Polyclonal RB25612 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis WB, IHC, FACS, IF, StM Host: Rat Monoclonal 9G10-F8-2 unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody
HSP90B1 Reactivity: Human, Mouse, Rat, Cow, Chicken, Hamster, Dog, Rabbit, Pig, Guinea Pig, Monkey, Sheep, Horse, Xenopus laevis IHC, FACS, StM Host: Rabbit Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibody
HSP90B1 Reactivity: Human, Mouse, Rat WB, ICC, IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 460-487) antibody
HSP90B1 Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB31597 unconjugated

Application Notes

Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:1000-1:10000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:1000-1:10000*

Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 , *endothelial cells

Restrictions

For Research Use only
Format

Liquid

Concentration

0.95 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Plate, Cooley, Chen, Paxman, Gallagher, Madoux, Genereux, Dobbs, Garza, Spicer, Scampavia, Brown, Rosen, Powers, Walter, Hodder, Wiseman, Kelly: "Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation." in: eLife, Vol. 5, (2017) (PubMed).
Target

GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name

GRP94 (HSP90B1 Products)

Synonyms

ecgp antibody, gp96 antibody, tra1 antibody, grp94 antibody, DDBDRAFT_0206450 antibody, DDBDRAFT_0215015 antibody, DDB_0206450 antibody, DDB_0215015 antibody, TRA1 antibody, HSP90B1 antibody, ERp99 antibody, GRP94 antibody, TA-3 antibody, Targ2 antibody, Tra-1 antibody, Tra1 antibody, endoplasmin antibody, GP96 antibody, fb61d09 antibody, tra-1 antibody, wu:fb61d09 antibody, wu:fq25g01 antibody, Grp94 antibody, ECGP antibody, Gp96 antibody, ppk98 antibody, hsp108 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, heat shock protein Hsp90 family protein antibody, 94 kD glucose-regulated protein antibody, heat shock protein 90 beta family member 1 antibody, heat shock protein 90, beta (Grp94), member 1 antibody, heat shock protein 90, beta (grp94), member 1 antibody, heat shock protein 90kDa beta family member 1 L homeolog antibody, hsp90b1 antibody, grp94 antibody, HSP90B1 antibody, Hsp90b1 antibody, hsp90b1.L antibody

Background

HSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. HSP90B1 is an endoplasmic reticulum HSP90 protein. Other HSP90 proteins are found in cytosol (see HSP90AA1, MIM 140571) and mitochondria (TRAP1, MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM]

Cellular Localization: Endoplasmic reticulum lumen , Melanosome

Molecular Weight

92 kDa

Gene ID

7184

Pathways

Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades