LIG3 antibody (C-Term)

Catalog No. ABIN2855575

Product Details anti-LIG3 Antibody

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG3 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Hamster, Human

Characteristics: Rabbit Polyclonal antibody to DNA ligase III (ligase III, DNA, ATP-dependent)
DNA ligase III antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase III. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: 293T

Validation: KO/KD, Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.15 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-LIG3 antibody (ABIN2855575)

Abdou, Poirier, Hendzel, Weinfeld: "DNA ligase III acts as a DNA strand break sensor in the cellular orchestration of DNA strand break repair." in: Nucleic acids research, Vol. 43, Issue 2, pp. 875-92, (2015) (PubMed).

Target Details for LIG3

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: DNA ligase 3 (LIG3 Products)

Background: This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Cellular Localization: Nucleus

Molecular Weight: 113 kDa

Gene ID: 3980

UniProt: P49916

Pathways: DNA Damage Repair