HSD3B2 antibody

Catalog No. ABIN2855488

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Product Details anti-HSD3B2 Antibody

Target: HSD3B2 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 2 (HSD3B2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD3B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to HSD3B2 (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2)
HSD3B2 antibody [N3C3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSD3B2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.24 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HSD3B2 antibody (ABIN2855488)

Qi, Liu, Zhang, Guo: "Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells." in: Scientific reports, Vol. 10, Issue 1, pp. 1281, (2020) (PubMed).

Target Details for HSD3B2

Target: HSD3B2 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 2 (HSD3B2))

Alternative Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 (HSD3B2 Products)

Synonyms: HSD3B antibody, HSDB antibody, SDR11E2 antibody, Hsd3b1 antibody, 3b-HSD antibody, HSD3B1 antibody, hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2 antibody, HSD3B2 antibody, Hsd3b2 antibody

Background: The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.

Cellular Localization: Endoplasmic reticulum membrane , Mitochondrion membrane

Molecular Weight: 42 kDa

Gene ID: 3284

UniProt: P26439

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis