WTAP antibody (Center)

Catalog No. ABIN2855463

Product Details anti-WTAP Antibody

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WTAP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Rhesus Monkey

Cross-Reactivity (Details): Mouse (100 %), Rat (100 %), Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to WTAP (Wilms tumor 1 associated protein)
WTAP antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human WTAP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: Molt-4

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for WTAP

Target: WTAP (Wilms Tumor 1 Associated Protein (WTAP))

Alternative Name: WTAP (WTAP Products)

Background: The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform.

Cellular Localization: Nucleus , nucleolus

Molecular Weight: 44 kDa

Gene ID: 9589