POLR2B antibody (N-Term)

Catalog No. ABIN2855420

Product Details anti-POLR2B Antibody

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Rat (Rattus), Rhesus Monkey, Cow (Bovine)

Cross-Reactivity (Details): Rat (100 %), Rhesus Monkey (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to RNA polymerase IIB (DNA-directed RNA polymerase II subunit RPB2)
RPB2 antibody [N1], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human RPB2. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: H1299 , BCL-1

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.65 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-POLR2B antibody (ABIN2855420)

Jönsson, Ludvik Brattås, Gustafsson, Petri, Yudovich, Pircs, Verschuere, Madsen, Hansson, Larsson, Månsson, Meissner, Jakobsson: "Activation of neuronal genes via LINE-1 elements upon global DNA demethylation in human neural progenitors." in: Nature communications, Vol. 10, Issue 1, pp. 3182, (2019) (PubMed).

Target Details for POLR2B

Target: POLR2B (Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa (POLR2B))

Alternative Name: RPB2 (POLR2B Products)

Synonyms: Pol2rb antibody, Rpb140 antibody, Rpb2 antibody, POL2RB antibody, RPB2 antibody, hRPB140 antibody, hsRPB2 antibody, RNA polymerase II subunit B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa antibody, DNA-directed RNA polymerase II subunit RPB2 antibody, polymerase (RNA) II (DNA directed) polypeptide B antibody, polymerase (RNA) II (DNA directed) polypeptide B, 140kDa L homeolog antibody, POLR2B antibody, polr2b antibody, LOC100640211 antibody, Polr2b antibody, polr2b.L antibody, rpb-2 antibody

Background: This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Cellular Localization: Nucleus

Molecular Weight: 134 kDa

Gene ID: 5431

Pathways: Regulatory RNA Pathways, DNA Damage Repair