NGFR antibody

Catalog No. ABIN2855341

Product Details anti-NGFR Antibody

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to p75 NGF Receptor (nerve growth factor receptor (TNFR superfamily, member 16))
p75 NGF Receptor antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human p75 NGF Receptor / CD271. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. FACS: Assay dependent. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: PC-12 , mouse brain , rat brain

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.43 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-NGFR antibody (ABIN2855341)

Zhang, Zhang, Luo, Yan, Niibe, Egusa, Zhang, Xie, Jiang: "Investigate the Odontogenic Differentiation and Dentin-Pulp Tissue Regeneration Potential of Neural Crest Cells." in: Frontiers in bioengineering and biotechnology, Vol. 8, pp. 475, (2020) (PubMed).

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: nerve growth factor receptor (NGFR Products)

Background: Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.

Cellular Localization: Membrane, Single-pass type I membrane protein

Molecular Weight: 45 kDa

Gene ID: 4804

UniProt: P08138

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding