HYOU1 antibody (C-Term)

Catalog No. ABIN2855339

Product Details anti-HYOU1 Antibody

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HYOU1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Plant, Rat

Characteristics: Rabbit Polyclonal antibody to ORP150 (hypoxia up-regulated 1)
ORP150 antibody [C2C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ORP150. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: mouse liver , rat brain , MCF-7 , MDA-MB-231

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.41 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for HYOU1

Target: HYOU1 (Hypoxia Up Regulated 1 (HYOU1))

Alternative Name: hypoxia up-regulated 1 (HYOU1 Products)

Synonyms: GRP-170 antibody, Grp170 antibody, HSP12A antibody, ORP-150 antibody, ORP150 antibody, AI415631 antibody, CBP-140 antibody, Cab140 antibody, Orp150 antibody, id:ibd5056 antibody, wu:fd14c05 antibody, zgc:56019 antibody, HYOU1 antibody, orp150 antibody, GRP170 antibody, hypoxia up-regulated 1 antibody, hypoxia up-regulated protein 1 antibody, hypoxia up-regulated 1 S homeolog antibody, HYOU1 antibody, Hyou1 antibody, hyou1 antibody, LOC100380749 antibody, hyou1.S antibody

Background: The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal.

Cellular Localization: Endoplasmic reticulum lumen

Molecular Weight: 111 kDa

Gene ID: 10525

UniProt: Q9Y4L1

Pathways: ER-Nucleus Signaling, SARS-CoV-2 Protein Interactome