KAP1 antibody

Catalog No. ABIN2855331

Product Details anti-KAP1 Antibody

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to KAP1 (tripartite motif containing 28)
KAP1 antibody [N3C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human KAP1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.48 mg/mL

Buffer: 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KAP1 antibody (ABIN2855331)

Liu, Li, Liu, Li, Wei, Liu, Lv: "Expression and Significance of TRIM 28 in Squamous Carcinoma of Esophagus." in: Pathology oncology research : POR, Vol. 25, Issue 4, pp. 1645-1652, (2020) (PubMed).

Hung, Johnson, Chen, Byrum, Bredemeyer, Yewdell, Johnson, Lee, Deivasigamani, Hindi, Amatya, Gross, Paull, Pisapia, Chaudhuri, Petrini, Mosammaparast, Amarasinghe, Zha, Tyler, Sleckman: "MRI Is a DNA Damage Response Adaptor during Classical Non-homologous End Joining." in: Molecular cell, Vol. 71, Issue 2, pp. 332-342.e8, (2019) (PubMed).

Wang, Wolgemuth: "BET Protein BRDT Complexes With HDAC1, PRMT5, and TRIM28 and Functions in Transcriptional Repression During Spermatogenesis." in: Journal of cellular biochemistry, Vol. 117, Issue 6, pp. 1429-38, (2016) (PubMed).

Aslanian, Yates, Hunter: "Mass spectrometry-based quantification of the cellular response to methyl methanesulfonate treatment in human cells." in: DNA repair, Vol. 15, pp. 29-38, (2014) (PubMed).

Target Details for KAP1

Target: KAP1 (TRIM28) (Tripartite Motif Containing 28 (TRIM28))

Alternative Name: tripartite motif containing 28 (TRIM28 Products)

Synonyms: KAP1 antibody, RNF96 antibody, TF1B antibody, TIF1B antibody, TRIM28 antibody, kap1 antibody, rnf96 antibody, tf1b antibody, tif1b antibody, AA408787 antibody, KAP-1 antibody, KRIP-1 antibody, Tif1b antibody, Tif1beta antibody, Kap1 antibody, Krip1 antibody, TIF1BETA antibody, tripartite motif containing 28 antibody, tripartite motif containing 28 L homeolog antibody, tripartite motif-containing 28 antibody, TRIM28 antibody, trim28.L antibody, Trim28 antibody

Background: The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

Cellular Localization: Nucleus (By similarity)

Molecular Weight: 89 kDa

Gene ID: 10155

UniProt: Q13263

Pathways: Hedgehog Signaling, Positive Regulation of Response to DNA Damage Stimulus