POM121 antibody (N-Term)

Catalog No. ABIN2855297

Product Details anti-POM121 Antibody

Target: POM121 (POM121 Membrane Glycoprotein (POM121))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POM121 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to POM121 (POM121 membrane glycoprotein (rat))
POM121 antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human POM121. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A549

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.16 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-POM121 antibody (ABIN2855297)

Ma, Li, Jia, Gong, Wang, Zhang, Gu, Tang: "POM121 is identified as a novel prognostic marker of oral squamous cell carcinoma." in: Journal of Cancer, Vol. 10, Issue 19, pp. 4473-4480, (2019) (PubMed).

Rodriguez-Bravo, Pippa, Song, Carceles-Cordon, Dominguez-Andres, Fujiwara, Woo, Koh, Ertel, Lokareddy, Cuesta-Dominguez, Kim, Rodriguez-Fernandez, Li, Gordon, Hirschfield, Prats, Reddy, Fatatis et al.: "Nuclear Pores Promote Lethal Prostate Cancer by Increasing POM121-Driven E2F1, MYC, and AR Nuclear Import. ..." in: Cell, Vol. 174, Issue 5, pp. 1200-1215.e20, (2019) (PubMed).

Jiang, Gan, Yen, Moussaud, McLean, Dickson: "Proaggregant nuclear factor(s) trigger rapid formation of α-synuclein aggregates in apoptotic neurons." in: Acta neuropathologica, Vol. 132, Issue 1, pp. 77-91, (2017) (PubMed).

Schwartz, Travesa, Martell, Forbes: "Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin." in: Nucleus (Austin, Tex.), Vol. 6, Issue 1, pp. 40-54, (2015) (PubMed).

Chow, Elgort, Dasso, Powers, Ullman: "The SUMO proteases SENP1 and SENP2 play a critical role in nucleoporin homeostasis and nuclear pore complex function." in: Molecular biology of the cell, Vol. 25, Issue 1, pp. 160-8, (2014) (PubMed).

Target Details for POM121

Target: POM121 (POM121 Membrane Glycoprotein (POM121))

Alternative Name: POM121 transmembrane nucleoporin (POM121 Products)

Synonyms: P145 antibody, POM121A antibody, 2610027A18Rik antibody, C80273 antibody, mKIAA0618 antibody, POM121C antibody, POM121 antibody, wu:fa16c07 antibody, wu:fl35g12 antibody, POM121 transmembrane nucleoporin antibody, nuclear pore membrane protein 121 antibody, POM121 transmembrane nucleoporin C-like antibody, POM121 transmembrane nucleoporin C antibody, nuclear envelope pore membrane protein POM 121C antibody, POM121 membrane glycoprotein b antibody, POM121 antibody, Pom121 antibody, POM121CL antibody, POM121C antibody, pom121 antibody, LOC100604279 antibody, pom121-b antibody

Background: The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. This gene encodes a member of the FG-repeat-containing nucleoporins. The protein encoded by this gene is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only one has been determined.

Cellular Localization: Nucleus , nuclear pore complex , Nucleus membrane, Single-pass membrane protein , Endoplasmic reticulum membrane, Single-pass membrane protein (By similarity)

Molecular Weight: 128 kDa

Gene ID: 9883

UniProt: Q96HA1