TRIM27 antibody

Catalog No. ABIN2855268

Product Details anti-TRIM27 Antibody

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM27 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to TRIM27 (tripartite motif containing 27)
TRIM27 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human TRIM27. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: K562 , THP-1 , HL-60 , U87-MG , SK-N-SH , IMR32 , rat testis

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.66 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for TRIM27

Target: TRIM27 (Tripartite Motif Containing 27 (TRIM27))

Alternative Name: tripartite motif containing 27 (TRIM27 Products)

Synonyms: RFP antibody, RNF76 antibody, AW538890 antibody, Gm19403 antibody, Rfp antibody, TRIM27 antibody, Rfp1 antibody, tripartite motif containing 27 antibody, tripartite motif-containing 27 antibody, TRIM27 antibody, Trim27 antibody

Background: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein.

Cellular Localization: Nucleus (By similarity) , Cytoplasm (By similarity)

Molecular Weight: 58 kDa

Gene ID: 5987

UniProt: P14373