PHGDH antibody (N-Term)

Catalog No. ABIN2855237

Product Details anti-PHGDH Antibody

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PHGDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to PHGDH (phosphoglycerate dehydrogenase)
PHGDH antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human PHGDH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , A431 , HeLaS3 , HeLa

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.87 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PHGDH

Target: PHGDH (phosphoglycerate Dehydrogenase (PHGDH))

Alternative Name: phosphoglycerate dehydrogenase (PHGDH Products)

Synonyms: fb38f06 antibody, zgc:65956 antibody, wu:fb38f06 antibody, embryo sac development arrest 9 antibody, F10M10.7 antibody, ECK2909 antibody, JW2880 antibody, 3-PGDH antibody, 3PGDH antibody, 4930479N23 antibody, A10 antibody, PGAD antibody, PGD antibody, PGDH antibody, SERA antibody, PDG antibody, phosphoglycerate dehydrogenase antibody, D-3-phosphoglycerate dehydrogenase antibody, 3-phosphoglycerate dehydrogenase antibody, phgdh antibody, EDA9 antibody, serA antibody, Sthe_2284 antibody, Dacet_1260 antibody, Mrub_0173 antibody, MMAH_RS07650 antibody, Arnit_0487 antibody, Ndas_0174 antibody, Mesil_2386 antibody, Slip_0010 antibody, Trad_0302 antibody, Acear_0027 antibody, Mahau_0665 antibody, MZHIL_RS05165 antibody, Mesop_1331 antibody, Thein_0855 antibody, Phgdh antibody, PHGDH antibody

Background: 3-Phosphoglycerate dehydrogenase (PHGDH, EC 1.1.1.95) catalyzes the transition of 3-phosphoglycerate into 3-phosphohydroxypyruvate, which is the first and rate-limiting step in the phosphorylated pathway of serine biosynthesis, using NAD+/NADH as a cofactor.[supplied by OMIM]

Molecular Weight: 57 kDa

Gene ID: 26227

UniProt: O43175

Pathways: Metabolism of Steroid Hormones and Vitamin D, Warburg Effect