PRKCSH antibody

Catalog No. ABIN2855219

Product Details anti-PRKCSH Antibody

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKCSH antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to PRKCSH (protein kinase C substrate 80K-H)
PRKCSH antibody [N1C1]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Recombinant protein encompassing a sequence within the center region of human PRKCSH. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , 293T

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.94 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for PRKCSH

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Alternative Name: protein kinase C substrate 80K-H (PRKCSH Products)

Background: This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum (ER). This protein is an acidic phospho-protein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease (PCLD). Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Cellular Localization: Endoplasmic reticulum

Molecular Weight: 59 kDa

Gene ID: 5589

UniProt: P14314

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process