HNRNPD/AUF1 antibody

Catalog No. ABIN2855184

Product Details anti-HNRNPD/AUF1 Antibody

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPD/AUF1 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit Polyclonal antibody to AUF1 (heterogeneous nuclear ribonucleoprotein D (AU-rich element RNA binding protein 1, 37 kDa))
AUF1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:5000-1:20000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , 293T , Raji

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.45 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HNRNPD/AUF1 antibody (ABIN2855184)

Hwang, Wagley, Law, Wei, Loh et al.: "Phosphorylation of poly(rC) binding protein 1 (PCBP1) contributes to stabilization of mu opioid receptor (MOR) mRNA via interaction with AU-rich element RNA-binding protein 1 (AUF1) and poly A ..." in: Gene, Vol. 598, pp. 113-130, (2017) (PubMed).

Target Details for HNRNPD/AUF1

Target: HNRNPD/AUF1 (HNRNPD) (Heterogeneous Nuclear Ribonucleoprotein D (HNRNPD))

Alternative Name: heterogeneous nuclear ribonucleoprotein D (HNRNPD Products)

Synonyms: AUF1 antibody, AUF1A antibody, HNRPD antibody, P37 antibody, hnRNPD0 antibody, Auf1 antibody, Hnrnpd antibody, HNRNPD antibody, p37 antibody, auf1a antibody, hnrpd antibody, hnrnpd0 antibody, C230004L04 antibody, Hnrpd antibody, wu:fa28b06 antibody, zgc:162951 antibody, heterogeneous nuclear ribonucleoprotein D antibody, heterogeneous nuclear ribonucleoprotein D L homeolog antibody, HNRNPD antibody, Hnrnpd antibody, hnrnpd antibody, hnrnpd.L antibody

Background: This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants.

Cellular Localization: Nucleus

Molecular Weight: 38 kDa

Gene ID: 3184

UniProt: Q14103